ABSTRACT
This report aims to expose the path and the results of the adaptation, in 2020, of two classes of the Letters Course at the University of Sao Paulo to the needs imposed by the suspension of face-to-face classes due the Covid-19 pandemic. In the report, it is narrated that, inspired by Paulo Freire's pedagogical assumptions, particularly his dialogical perspective of teaching, attempts were made to create channels of communication with the students, having been adopted, after some of these attempts, a format that included not only online classes, but also asynchronous moments provided by sending study and analysis materials in order to respect a new reality imposed by social isolation. Positive results are described in the teacher-trainee-student interaction and in the student-student interaction. It is expected to contribute to the debate about the forms of education in the pandemic.
ABSTRACT
Introduction: While Covid-19 pandemic spreads around the world, there has been described different evolution in individuals according to risk groups. The onset of the disease, which is mainly associated with respiratory distress, has its classic imaging presentation as ground-glass opacities in computer tomography (CT), although there are plenty of other possible findings. In pediatrics, the infection has its own particularities, rarely presenting severe course. Since April 2020, even if uncommon and less prevalent than in adults, cases with worse outcome started being described, mainly associated with multisystem inflammatory syndrome. Oncologic patients have higher mortality rates, and in the hematologic malignancies, this risk factor is even more evident, where severe lymphopenia is frequent. Proportionally, there is still small data about pediatric leukemia patients during Covid-19 disease, as there is also lack of imaging descriptions. Objectives: Evaluation of pediatric patients under leukemia treatment that had Sars Cov 2 infection, correlating clinical and radiologic presentations. Material and methods: Data was collected from patients in the institution. Leukemia patients with molecular confirmation of Covid-19 were considered. Patients that didn't have confirmation of infection on RT-PCR test were excluded from analysis. Results: During the period from March 2020 to May 2021, there were 11 subjects with leukemia that had Sars Cov 2 proved infection. They were from 3 to 17 years old at Covid-19 diagnosis. From those, 6 had B ALL, 1 T ALL and 4 AML. 1 AML patient had previous allogeneic stem cell transplant. 6 patients had lymphocytes count < 550/mL. 6 patients underwent CT (5 with lymphopenia), and all presented radiologic signs. The main presentation was ground-glass opacity (GGO), present on all the 6 CTs. GGO was bilateral in 4 patients. The other findings were nodule (1), consolidation (1), interlobular septal thickening (1). The patient that presented consolidation had it localized, unilateral and perihilar. Regarding clinical evolution, 5 patients were classified as critical and transferred to ICU;all had a CT with GGO. 3 of them needed respiratory support and 1 needed only supplementary oxygen. This one had an atypical presentation, firstly suspected of transfusion-related acute lung injury, supported by clinical history. 2 patients had septic shock, renal failure and myocarditis combined. There was 1 decease (mortality 9% in the group), in a patient with severe respiratory presentation, associated with pulmonary Graft-versus-host disease. Discussion: This study showed that the main imaging finding in the group was GGO, which was present in all patients that had a CT performed. All the subjects that needed intensive care had imaging proof of pulmonary involvement, even if the complications were also due to multisystemic inflammation. CT has been described with a high sensitivity for symptomatic Covid-19, and when it is very typical, can be diagnostic also if a negative RT-PCR. Patients undergoing leukemia treatment are immunocompromised and have low anti-viral defense, which could lead to higher risk of worse onset. The exam was not done for all patients in this study, the choice for indicating CT was based on clinic presentation and laboratory, which could bring a bias on positivity for GGO. Conclusion: GGO is the main finding in CT scan in Covid-19, which was also found in this pediatric leukemia group.
ABSTRACT
Coronavirus Acute Respiratory Syndrome (SARS-CoV-2) is a very recent viral infection and has generated one of the world's biggest problems of all time. There is no scientific evidence and clinical trials to indicate that possible therapies have shown results in suspected or confirmed patients other than the use of immunizations. Given the above, some substances are being studied to be applied to contain their spread and further damage. This work aims to perform an in silico study of amoxicillin, widely known as an antibiotic and used to prevent bacterial infections and a possible biomarker made from a complex with Europium (Eu). It was shown to have the ability to interact with the COVID-19 protein in Mproprotease as ligands. The study was conducted using the AutoDock Vina with Lamarckian genetic model algorithm (GA) combined with the estimation of grid-based energy in rigid and flexible conformation. Compared to affinity energy, amoxicillin presented â-7.7kcal/mol, which was better than its co-crystallized ligand in the study. The Europium complex, where its synthesis was also demonstrated in this work, presented energy of -6.3kcal/mol with hydrogen bonds and possible color change when UV light was applied. For the choice of the best poses in the simulation, the neural network parameter, NNScore2, was used. It can be affirmed that this study is still introductory but promising both in the treatment and identification of the virus. © 2021 Journal of Computational Biophysics and Chemistry. All Rights Reserved.